Prepare and stain a root tip squash to observe the stages of mitosis OBJECTIVES ● To know how to prepare a temporary slide of a root tip to observe mitosis ● To recognise the stages of mitosis in dividing cells ● To identify hazards, associated risks and control...
Practical Skills
Core Practical 7
Use a light microscope to: make observations of (i) transverse sections of roots, stems, leaves; (ii) plant tissues; (iii) identify sclerenchyma fibres, phloem, sieve tubes, xylem vessels and their location OBJECTIVES ● To be able to use a microscope competently to...
Core Practical 8
Determine the tensile strength of plant fibres OBJECTIVES ● To know how to determine the tensile strength of plant fibres ● To be able to comment on experimental design and evaluate scientific methods SAFETY ● Use a sharp knife, place the celery on the tile and cut...
Core Practical 9
Investigate the antimicrobial properties of plants, including aseptic techniques for the safe handling of bacteria OBJECTIVES ● To successfully compare the effect of plants on microbial growth ● To understand the safety issues of microbiological work and how to apply...
Core Practical 4
Investigate the effect of temperature, pH, enzyme concentration and substrate concentration on the initial rate of enzyme-catalysed reactions. OBJECTIVES ● To be able to measure the initial rate of enzyme activity ● To understand why measuring the initial rate is...
Core Practical 5
(i) Use a light microscope to make observations and labelled drawings of suitable animal cells; (ii) Use a graticule with a microscope to make measurements and understand the concept of scale OBJECTIVES ● To be competent in the use of a microscope at high and low...
Core Practical 2
Investigate the vitamin C content of food and drink. OBJECTIVES ● To be able to calculate the vitamin C concentration of fruit juices using the titration method ● To solve problems set in practical contexts ● To process and analyse data using appropriate mathematical...
Core Practical 3
Investigate membrane properties including the effect of alcohol and temperature on membrane permeability. OBJECTIVES ● To know how the effect of temperature and alcohol on membranes can be determined ● To be able to recognise quantitative variables that should be...
Core Practical 1
Use a semi-quantitative method with Benedict's reagent to estimate the concentrations of reducing sugars and with iodine solution to estimate the concentrations of starch, using colour standards. OBJECTIVES ● To understand what is meant by a semi-quantitative test ●...
