Actual object size = Image size/magnification I=AxM
Practical:
- 1. prepare your slide – add drop of water, specimen, iodine then coverslip
- 2. clip slide onto stage, select lowest power objective lens, use coarse adjustment knob, adjust focus with fine adjustment knob
- 3. draw observations (in pencil), write title, magnification, label important features
Electron microscopes have a higher resolution
Iodine is needed to stain the sample to highlight objects in the cell by adding colour