Helicase Unwinds parental double helix at replication forks
Single-stranded binding protein Binds to, stabilizes, and prevents single-stranded DNA from rejoining until is is used as a template
Topoisomerase Binds ahead of replication forks & relieves overwinding strain by breaking, swiveling, and rejoining DNA strands
Primase Synthesizes an RNA primer at 5’ end of leading strand and at 5’ end of each okazaki fragment
DNA Ligase Joins okazaki fragments w/ covalent bonds; on leading strand joins 3’ end of DNA that replaces primer to rest of leading strand DNA