- The Beer-Lambert law relates the absorption of light by a solution to three variables according to the equation
- We can determine the concentration (molarity) of a sample by looking at the absorbance
Factors Affecting Calculations
- Dilution (left water)→ measured absorbance is too low
- Dirtiness (Ex: left fingerprints) or path of light is obstructed (ex: frosted wall)→ less light would pass thru solution and be detected = more light absorbed